Using Deep Mutational Scanning to Generate a Complete Map of SARS-CoV-2 Diagnostic Antibody Escape Mutations
To evaluate the impact of variant mutations on rapid antigen tests our speakers developed a mammalian surface-display platform for SARS-CoV-2 Nucleocapsid protein, the target of the majority of antigen tests. They employed deep mutational scanning to evaluate binding of all possible Nucleocapsid point mutations to diagnostic antibodies. This approach generates unique escape mutation profiles for each antibody which serve as a valuable resource for predicting performance of antigen tests against past, current, as well as future variants of SARS-CoV-2.
When
April 19, 2022
2:00 PM ET
Speakers
Filipp Frank, PhD, Department of Biochemistry, Emory University
Eric Ortlund, PhD, Professor, Department of Biochemistry, Emory University